Principal Investigators

Friedrich ALTMANN is Associate Professor at the Department of Chemistry. His research interest is focused on glycoproteins and the various roles of their sugar moities. Particular topics are the biosynthesis, structure and function of protein-bound glycans in expression systems for recombinant proteins; structural analysis of oligosaccharides from mammalian glycoproteins but also from plants and others; the role of non-mammalian protein-linked glycans as immunogens and allergens; proteomic and glyco-proteomic analysis of biotechnological products; development of the methodology for "glycomic" analysis of glycoprotein glycans by mass spectrometry (LC-ESI-MS/MS).
Projects within BioToP
Email: Friedrich Altmann
Institute Homepage: Group Altmann

Nicole Borthis Associated Professor at the Department of Biotechnology. Her research aims at obtaining a comprehensive understanding of the molecular machinery that enables a cell to be a good production factory for biotherapeutic proteins. This includes factors that contribute to high yield based on productivity and growth, but also to protein quality attributes. The methods used are transcriptomics, miRNA arrays, proteomics and flow cytometry / cell sorting. The goal is to understand the molecular basis and regulation of such process and quality relevant cellular properties. The obtained knowledge can be used in different ways, (i) for the development of new screening approaches using cell sorting and relevant molecular markers, (ii) for targeted cell engineering to improve the cellular machinery, (iii) for process monitoring and control based on the prediction of product quality from the state of the producing cell.
Projects within BioToP
Email: Nicole Borth
Institute Homepage: Group Borth

Reingard GRABHERR is Associate Professor at the Department of Biotechnology. Her main focus is directed towards protein expression design, cellular engineering and systems biology approaches for the production of functional proteins, virus like particles and infectious viruses. Her goals are influenza virus vaccine design in insect cells and mammalian cells as well as improving cell factories in general for production of functional proteins (e.g. enzymes) and DNA (DNA vaccine). Her core competence is design of expression cassettes and genetic engineering of production strains, needed for pharmaceutical and industrial biotechnology. Her special interest lies in improving surface display techniques in insect and mammalian cells using recombinant baculoviruses, exploring different strategies for display of functional proteins, expression of eukaryotic complex and multimeric proteins in insect cells, comparing the insect cell and yeast system in terms of display efficiency and authenticity of eukaryotic proteins and the development of cloning strategies for large and diverse protein libraries in insect and mammalian cells.
Projects within BioToP
Email: Reingard Grabherr
Institute Homepage:

Johannes GRILLARI is Associate Professor at the Department of Biotechnology. His research interests include: Functional analyses of various primary human cells like umbilical vein endothelial cells, fibroblasts and epithelial kidney cells, but also of CHO cell lines with emphasis on replicative life span and stress resistance. Identification of differentially expressed genes and proteins in these cell lines. Functional characterization of thereby identified miRNAs and proteins in the context of cell proliferation, senescence and stress resistance.
Projects within BioToP
Email: Johannes Grillari
Institute Homepage: Group Grillari

Dietmar HALTRICH is Professor at the Department of Food Science and Technology. His research interests include: Biochemistry of flavoproteins of the GMC family of oxidoreductases (specifically pyranose oxidase, pyranose dehydrogenase, cellobiose dehydrogenase) and copper-containing oxidoreductases; glycosidases and glycosyl transfer reactions catalysed by these enzymes; biocatalytic production of food-related carbohydrates including prebiotic sugars (galacto-oligosaccharides, tagatose); enzyme engineering and evolution; tailoring of enzymes for biofuel cell applications.
Projects within BioToP
Email: Dietmar Haltrich
Institute Homepage: Group Haltrich

Alois JUNGBAUER is Associate Professor at the Department of Biotechnology. His research interests include: Biochemical engineering sciences, in particular in bioseparation and protein technology; activities range from protein expression and downstream processing with emphasis on scale-up, continuous processing and fundamental understanding of fixed bed processes; studies in the field of bioengineering of proteins, plasmids and viruses with special focus on expression, downstream processing and characterization of large biomolecules; development of novel concepts for separation of large biomolecules and characterization of their biophysical properties.
Projects within BioToP
Email: Alois Jungbauer
Institute Homepage: Group Jungbauer

Renate KUNERT is Professor at the Department of Biotechnology. Her research interests include: Methodological platforms for the sophisticated and efficient development of stable recombinant mammalian cell lines expressing complex and membrane proteins; vector optimization for various host systems and protein families; optimization of protein maturation to reduce cellular stress; screening and characterization of transfectants regarding cellular membrane functionality and cellular physiology in different host cells in serum-free systems; effects of ligand induced internalization on cell physiology during expression of membrane proteins in stable recombinant cell lines; investigation of receptor associated cellular membrane rearrangement.
Projects within BioToP
Email: Renate Kunert
Institute Homepage: Group Kunert

Lukas MACH is Associate Professor at the Department of Applied Genetics and Cell Biology. His research interests include: Biosynthesis and intracellular trafficking of secretory and lysosomal/vacuolar proteins, post-translational protein modifications as determinants of protein structure and function, physiological functions of proteinases and their inhibitors, recombinant expression of therapeutically relevant proteins in plants.
Projects within BioToP
Email: Lukas Mach
Institute Homepage: Group Mach

Diethard MATTANOVICH is Professor for Microbial Cell Design at the Department of Biotechnology. His research interests include: Physiology and genetics of microbial cell factories; production of heterologous proteins in yeasts (complex biopharmaceutical proteins and industrial enzymes); systems biotechnology; stress regulation in yeast, related to environmental and metabolic stressors; metabolic and cell engineering for improved productivity and stress resistance.
Projects within BioToP
Email: Diethard Mattanovich
Institute Homepage: Group Mattanovich

Christian OBINGER is Professor for Biochemistry at the Department of Chemistry. His research interests include: Biochemistry and structure-function analysis of redox-active metalloenzymes with focus on heme oxidoreductases (peroxidases, catalases, cytochromes and terminal respiratory oxidases); mechanisms, thermodynamics and kinetics of one- and two-electron transfer reactions; role of post-translational polypeptide and heme modifications in catalysis of oxidoreductases; protein radical formation and migration in heme proteins; enzyme-mediated halogenation and nitration reactions; thermodynamics and kinetics of protein unfolding and folding.
Projects within BioToP
Email: Christian Obinger
Institute Homepage: Group Obinger

Chris OOSTENBRINK is professor for Biomolecular Modeling and Simulation. His research interests include: Computational methods to describe the structure, function and dynamics of biomolecular systems; development of accurate classical force fields; efficient calculation of binding free energies of a series of compounds to a common macromolecular target; method development and practical applications to rationalize and predict experiments; enhanced sampling techniques to address conformational changes and protein folding problems; Effect of protein flexibility on function and behaviour.
Projects within BioToP
Email: Chris Oostenbrink
Institute Homepage: Group Oostenbrink

Herta STEINKELLNER is Associate Professor at the Department of Applied Genetics and Cell Biologiy. She is leading the research group Molecular Plant Biotechnology. Her main interest is the development of plant based expression systems that allow the generation of valuable proteins with a customised glycosylation profile and is recognized by her pioneering work in the generation of plant glycosylation mutants that enable human type protein glycosylation. She is particularly proud of the recently granted Laura Bassi Center of Expertise "Production of Glycan Optimised Biopharmaceuticals in Plants". During her scientific career she worked at international renovated research institutes like Scripps Research Institute, USA; Osaka University, JP and John Innes Plant Science Institute, UK.
Projects within BioToP
Email: Herta Steinkellner
Institute Homepage: Group Steinkellner

Eva STÖGER is Professor at the Department of Applied Genetics and Cell Biology. Her research interests include: Crop biotechnology and molecular farming; recombinant production and analysis of pharmaceutically or nutritionally relevant proteins (with emphasis on monoclonal antibodies) in different plant species, including tobacco, medicago and cereals; biochemical, structural and functional characterisation of the products. A main focus involves the use of plant seeds for high-yield and stable accumulation of recombinant proteins. Several studies have been carried out to explore the deposition of endogenous and recombinant proteins in storage organelles and to utilize these intracellular compartments for the accumulation of recombinant enzymes and pharmaceutical proteins.
Projects within BioToP
Email: Eva Stöger
Institute Homepage: Group Stöger

Iain WILSON is Associate Professor at the Department of Chemistry. His research interests include: Molecular biology and biochemistry of enzymes related to glycosylation and relevant glycan structures, whether from invertebrates, vertebrates, plants or protozoa; antibody and lectin binding by glycans from invertebrates, plants and protozoa; determination of ‘glycozyme’ substrate specificities and associated ‘molecular phenotypes’ using reverse genetic approaches (mutants, RNAi) and biochemical/analytical characterisation; examination of glycosylation mutants (knock-down or knock-out) for basic research (understanding of glycosylation pathways) or ‘translational’ research (optimisation of cell lines).
Projects within BioToP
Email: Iain Wilson
Institute Homepage: Group Wilson