Projects of Area 3 - Expression systems and cell factories List of ongoing PhD research projects: RESEARCH PROJECT SUPERVISOR PhD STUDENT Exploring energy metabolism on C1 carbon sources in the yeast Komagataella phaffii Diethard Mattanovich Daniel Ahlheit Grafting the ribulose monophosphate cycle for methanol assimilation into Komagataella phaffii Diethard Mattanovich Miriam Kuzman Plant-based production and bioencapsulation of immunotherapeutics for allergy treatment Eva Stöger Fabian Schubert Multi-omics analysis of CHO cell lines Nicole Borth Markus Riedl Synthetic biology-based cell engineering of the yeast Pichia pastoris Brigitte Gasser Pawel Malecki Enzyme engineering and evolution for improvement of methanol utilization in yeast Diethard Mattanovich Charles Moritz Identification of both coding and non-coding genomic regions that contribute to Bioprocess relevant behaviour Nicole Borth Ivy Rose Sebastian Effect of membrane organization on weak acid transport proteins Karola Vorauer-Uhl Yao Yuelang Design of a minimal enzyme set for lignin depolymerization Michael Sauer Silja Välimets Seasonal influenza virus vaccine effectiveness through recombinant neuraminidase on VLPs Reingard Grabherr Leticia Guzman Ruiz O-glycan engineering of N. benthamiana for structure-function characterization of human IgA1 Eva Stöger Pia Uetz Impact of cellular redox state on protein production and secretion in yeast Brigitte Gasser Victor Honorato Control of glycolysis in Crabtree-negative and positive yeasts Diethard Mattanovich Yoshua Donny Rudinatha Synthetic biology tools to control expression of multiple genes in a pathway Nicole Borth Caterina Ruggeri Pichia pastoris genetics for strain development Diethard Mattanovich Marina Jecmenica Establishment of a hyper-secreting eukaryotic protein production system as alternative cell factory for biotherapeutics Nicole Borth Michael Coats Defined gp140 trimers as HIV vaccine Renate Kunert Philipp Mundsperger List of completed PhD research projects: RESEARCH PROJECT SUPERVISOR PhD STUDENT Increasing efficiency of CO2 assimilation in synthetic autotrophic Pichia pastoris Diethard Mattanovich Michael Baumschabl Understand and exploit sigma-appropriation as a tool for transcription control in E. coli Reingard Grabherr Marco Klanschnig Designed protein storage organelles and their uptake into target cells Eva Stöger Jennifer Schwestka Inducible regulation of baculovirus genes involved in secretion and virus budding Reingard Grabherr Christina Hausjell Engineering and functional activities of polysialylated IgG antibodies Herta Steinkellner Lukas Eidenberger Advanced analytical strategies for investigation of alternative methanol assimilation in methylotrophic yeasts Diethard Mattanovich Bernd Mitic Introduction of unnatural amino acids in recombinant proteins with the yeast Pichia pastoris Diethard Mattanovich Nora Tir Protein-liposome conjugates as novel HIV vaccine candidates Karola Vorauer-Uhl Ehsan Suleiman Development of a seed-based strategy for the design of a mucosal vaccine against S. pneumoniae Eva Stöger Marc Tschofen Understanding molecular transport processes for improved metabolic engineering approaches Michael Sauer Dominik Jeschek Rational systems design of protein-producing yeasts: eliminating metabolic limitations Brigitte Gasser David Pena Productivity optimisation of CHO cells by epigenome alterations Nicole Borth Peter Eisenhut Quantitative metabolic flux analysis in CHO cells Nicole Borth Diana Szeliova Change of the Global Methylation Pattern to Alter the Growth Phenotype of CHO-CellsBackground Nicole Borth Marcus Weinguny Enzyme scaffolding and cellular compartments for metabolic engineering endeavours Michael Sauer Bernd Albrecht In silico guided humanization of antibodies Renate Kunert Linda Schwaigerlehner Cellular Mechanisms of Regulation Brigitte Gasser Sonakshi De Characterization of transcriptional regulatory proteins Brigitte Gasser Diane Barbay Immunoglobulin E (IgE) and glycosylation Herta Steinkellner Laura Montero Morales Understanding the source of variation in protein N-glycosylation patterns of recombinant proteins generated in CHO cells Nicole Borth Nina Bydlinski Characterization of microbial methanol metabolism Diethard Mattanovich Domen Zavec Metabolic engineering of Pichia pastoris Diethard Mattanovich Thomas Gassler Generation and presentation of membrane protein targets using influenza virus−like particles Reingard Grabherr Nika Lisa Understanding and controlling the expression of recombinant oligomeric immunoglobulins Renate Kunert Julia Hennicke Molecular engineering of baculoviral based protein expression in insect cells Reingard Grabherr Krisztina Koczka Genomic rearrangements in CHO producer cell lines Nicole Borth Inmaculada Hernandez-Lopez Engineering Pichia pastoris for antibody surface display Diethard Mattanovich Lina Heistinger Plant glyco-engineering towards synthesis of recombinant proteins with polysialylated N-glycans Herta Steinkellner Somanath Kallolimath Investigation and Enhancement of Growth Characteristics of Chinese Hamster Ovary Cells Nicole Borth Gerald Klanert Generation and presentation of membrane protein targets using baculovirus surface display Reingard Grabherr Monika Wilde Influenza virus−like particles as antigen carrier platform for conserved influenza viral epitopes Reingard Grabherr Miriam Klausberger Cross−Species Comparison of Expression Systems Reingard Grabherr Andreas Maccani Directed evolution of CHO host and production cell lines Johannes Grillari Eva Harreither MicroRNOMics of CHO host and production cell lines Johannes Grillari Vaibhav Jadhav Yeast Cell Wall Characterisation Gunda Köllensperger Evelyn Rampler Quantitative Metabolomics by LC–MS Gunda Köllensperger Karin Ortmayr Regulation of the expression of recombinant pentameric IgMs Renate Kunert Veronika Chromikova Powerful expression of recombinant proteins Renate Kunert Wolfgang Sommeregger Influence of amino acid sequence on growth and secretion of recombinant CHO cell lines Renate Kunert Patrick Mayrhofer Regulation of heterologous protein production in relation to cell growth Diethard Mattanovich Corinna Rebnegger Interrelation of core metabolism with the production of heterologous proteins in yeast Diethard Mattanovich Justyna Nocon Antibody production in seed tissues: evaluating the subcellular deposition, N−glycan modification and functionality Eva Stöger Stanislav Melnik Artificial storage organelles for the optimal production of biopharmaceuticals in plants and plant cells Eva Stöger Anna Hofbauer
