Projects of Area 3 - Expression systems and cell factories

List of ongoing PhD research projects:

Enzyme engineering and evolution for improvement of methanol utilization in yeast   Diethard Mattanovich Charles Moritz
Identification of both coding and non-coding genomic regions that contribute to Bioprocess relevant behaviour   Nicole Borth Ivy Rose Sebastian
Effect of membrane organization on weak acid transport proteins   Karola Vorauer-Uhl Yao Yuelang
Design of a minimal enzyme set for lignin depolymerization   Michael Sauer Silja Välimets
Seasonal influenza virus vaccine effectiveness through recombinant neuraminidase on VLPs   Reingard Grabherr Leticia Guzman Ruiz
O-glycan engineering of N. benthamiana for structure-function characterization of human IgA1   Richard Strasser Valentina Ruocco
Engineering of a bispecific mAb recognizing an optimized epitope of IgE as well as CD3 – a novel strategy for fighting allergic diseases   Florian Rüker Aleksandra Rodak
Redox communication between cytosol and ER   Brigitte Gasser Lukas Zeh
O-glycan engineering of N. benthamiana for structure-function characterization of human IgA1   Eva Stöger Pia Uetz
Impact of cellular redox state on protein production and secretion in yeast   Brigitte Gasser Victor Honorato
Advanced analytical strategies for investigation of alternative methanol assimilation in methylotrophic yeasts   Diethard Mattanovich Bernd Mitic
Control of glycolysis in Crabtree-negative and positive yeasts   Diethard Mattanovich Yoshua Donny Rudinatha
Inducible regulation of baculovirus genes involved in secretion and virus budding   Reingard Grabherr Christina Hausjell
Synthetic biology tools to control expression of multiple genes in a pathway   Nicole Borth Caterina Ruggeri
Understand and exploit sigma-appropriation as a tool for transcription control in E. coli   Reingard Grabherr Marco Klanschnig
Designed protein storage organelles and their uptake into target cells   Eva Stöger Jennifer Schwestka
Engineering and functional activities of polysialylated IgG antibodies   Herta Steinkellner Lukas Eidenberger
Pichia pastoris genetics for strain development   Diethard Mattanovich Marina Jecmenica
Introduction of unnatural amino acids in recombinant proteins with the yeast Pichia pastoris   Diethard Mattanovich Nora Tir
Establishment of a hyper-secreting eukaryotic protein production system as alternative cell factory for biotherapeutics   Nicole Borth Michael Coats
Defined gp140 trimers as HIV vaccine   Renate Kunert Philipp Mundsperger
Protein-liposome conjugates as novel HIV vaccine candidates   Karola Vorauer-Uhl Ehsan Suleiman

List of completed PhD research projects:
Development of a seed-based strategy for the design of a mucosal vaccine against S. pneumoniae   Eva Stöger Marc Tschofen
Understanding molecular transport processes for improved metabolic engineering approaches   Michael Sauer Dominik Jeschek
Rational systems design of protein-producing yeasts: eliminating metabolic limitations   Brigitte Gasser David Pena
Productivity optimisation of CHO cells by epigenome alterations   Nicole Borth Peter Eisenhut
Quantitative metabolic flux analysis in CHO cells   Nicole Borth Diana Szeliova
Change of the Global Methylation Pattern to Alter the Growth Phenotype of CHO-CellsBackground   Nicole Borth Marcus Weinguny
Enzyme scaffolding and cellular compartments for metabolic engineering endeavours   Michael Sauer Bernd Albrecht
In silico guided humanization of antibodies   Renate Kunert Linda Schwaigerlehner
Cellular Mechanisms of Regulation   Brigitte Gasser Sonakshi De
Characterization of transcriptional regulatory proteins   Brigitte Gasser Diane Barbay
Immunoglobulin E (IgE) and glycosylation   Herta Steinkellner Laura Montero Morales
Understanding the source of variation in protein N-glycosylation patterns of recombinant proteins generated in CHO cells   Nicole Borth Nina Bydlinski
Characterization of microbial methanol metabolism   Diethard Mattanovich Domen Zavec
Metabolic engineering of Pichia pastoris   Diethard Mattanovich Thomas Gassler
Generation and presentation of membrane protein targets using influenza virus−like particles   Reingard Grabherr Nika Lisa
Understanding and controlling the expression of recombinant oligomeric immunoglobulins   Renate Kunert Julia Hennicke
Molecular engineering of baculoviral based protein expression in insect cells     Reingard Grabherr Krisztina Koczka
Genomic rearrangements in CHO producer cell lines   Nicole Borth Inmaculada Hernandez-Lopez
Engineering Pichia pastoris for antibody surface display   Diethard Mattanovich Lina Heistinger
Plant glyco-engineering towards synthesis of recombinant proteins with polysialylated N-glycans   Herta Steinkellner Somanath Kallolimath
Investigation and Enhancement of Growth Characteristics of Chinese Hamster Ovary Cells   Nicole Borth Gerald Klanert
Generation and presentation of membrane protein targets using baculovirus surface display   Reingard Grabherr Monika Wilde
Influenza virus−like particles as antigen carrier platform for conserved influenza viral epitopes   Reingard Grabherr Miriam Klausberger
Cross−Species Comparison of Expression Systems   Reingard Grabherr Andreas Maccani
Directed evolution of CHO host and production cell lines   Johannes Grillari Eva Harreither
MicroRNOMics of CHO host and production cell lines   Johannes Grillari Vaibhav Jadhav
Yeast Cell Wall Characterisation   Gunda Köllensperger Evelyn Rampler
Quantitative Metabolomics by LC–MS   Gunda Köllensperger Karin Ortmayr
Regulation of the expression of recombinant pentameric IgMs   Renate Kunert Veronika Chromikova
Powerful expression of recombinant proteins   Renate Kunert Wolfgang Sommeregger
Influence of amino acid sequence on growth and secretion of recombinant CHO cell lines   Renate Kunert Patrick Mayrhofer
Regulation of heterologous protein production in relation to cell growth   Diethard Mattanovich Corinna Rebnegger
Interrelation of core metabolism with the production of heterologous proteins in yeast   Diethard Mattanovich Justyna Nocon
Antibody production in seed tissues: evaluating the subcellular deposition, N−glycan modification and functionality   Eva Stöger Stanislav Melnik
Artificial storage organelles for the optimal production of biopharmaceuticals in plants and plant cells   Eva Stöger Anna Hofbauer