Projects of Area 1 - Structure-function analysis, engineering and design of proteins List of ongoing PhD research projects: RESEARCH PROJECT SUPERVISOR PhD STUDENT Developing a framework to enhance T-cell receptor functionality Michael Traxlmayr Philipp Brunmayr Importance of heme biosynthesis in the oral pathogen Porphyromonas gingivalis Stefan Hofbauer Jakob Ender Biochemistry of actinobacterial coproporphyrin ferrochelatase from Corynebacterium diphtheriae Stefan Hofbauer Alice Cassiani Novel sialidases for enzymatic recovery of sialic acid from edible bird's nest (EBN) co-products Dietmar Haltrich Nur Afifah Binto Mohd Fauziee New enzymes, reconstruction and design for industrial synthesis Clemens Peterbauer Chiara Amoroso Engineering OR-gated CAR T cells to prevent resistance due to antigen loss Michael Traxlmayr Julia Mayer Elucidating the molecular mechanism of engineered binders targeting the activated conformation of EGFR Michael Traxlmayr Delia Sumesgutner Exploitation of transglycosylation activities of bifidobacterial glycoside hydrolases for the synthesis of human milk oligosaccharide (HMO) structures Dietmar Haltrich Konlarat Phirom-on Variation of the linking loop in dimeric α-β barrel proteins Stefan Hofbauer Nikolaus Falb Structural studies of actinobacterial coproheme decarboxylase (ChdC) from Corynebacterium diphtheriae Stefan Hofbauer Patil Gaurav Glycobiology aspects of Tannerella serpentiformis Christina Schäffer Stephanie Walcher Novel Lactate-Oxidizing Enzymes for Biosensor Applications Dietmar Haltrich Lidiia Tsvik Deciphering the folding and inhibition mechanism of the staphylococcal myeloperoxidase inhibitor SPIN Christian Obinger Urban Leitgeb Engineering a small molecule regulated heterodimerization system for the control of CAR T cells Christian Obinger Elise Sylvander Development of a novel binder scaffold Christian Obinger Magdalena Teufl List of completed PhD research projects: RESEARCH PROJECT SUPERVISOR PhD STUDENT Secondary cell wall polymer pyruvylation as an essential ligand modification for SLH-domain recognition Christina Schäffer Cordula Stefanovic Increasing thermostability of pyranose oxidase by enzyme evolution Dietmar Haltrich Anja Kostelac The cancer immunotherapy antigen CD19 and its molecular interaction network Christian Obinger Jacqueline Seigner Allosteric molecular recognition to improve tumor specificity Michael Traxlmayr Markus Dobersberger Engineering of a bispecific mAb recognizing an optimized epitope of IgE as well as CD3 – a novel strategy for fighting allergic diseases Florian Rüker Aleksandra Rodak Resolving dynamic protein conformations in multidomain enzymes with SAXS Roland Ludwig Bettina Motycka Biochemistry of firmicute coproporphyrin ferrochelatase from Listeria monocytogenes Christian Obinger Thomas Gabler Cytochromes as electron transfer mediators for bioelectrodes Roland Ludwig Lorenz Schwaiger Bispecific antibodies with monovalent antigen engagement in the Fc domain Florian Rüker Filippo Benedetti Routing the flow of electron from cytochromes to oxidoreductases Roland Ludwig Franziska Schachinger Accessory Activities 3: Novel Flavoprotein Oxidoreductase from bacterial sources Clemens Peterbauer Virginia Ludovika Biochemistry of actinobacterial coproheme decarboxylase (ChdC) from Corynebacterium diphteriae Christian Obinger Hanna Michlits Structural and mechanistic studies on chlorite dismutase (Cld) from Cyanothece sp. PCC7425 Christian Obinger Daniel Schmidt Structural characterization of Z-disk complex Dietmar Haltrich Antonio Sponga Development of high-throughput-screening of flavin- and heme-dependent oxidoreductases using genetically encoded redox sensors Clemens Peterbauer Peter Herzog Extracellular vesicles as tool for drug delivery Florian Rüker Stefan Vogt Mechanism and dynamics of extracellular cellulolytic protein interactions Roland Ludwig Christophe Laurent Structure−function analysis of a phylum-wide conserved glycosyltransferase central to a novel protein O−glycosylation pathway Christina Schäffer Matthias Braun Molecular basis of pyruvylated cell wall polymer – SLH-protein interactions Christina Schäffer Fiona Hager Engineering of T-cell receptor for bispecificity Florian Rüker Flavio Sadio Biochemistry of invertebrate peroxidasins Christian Obinger Benjamin Sevcnikar Developing rcSso7d-based chimeric antigen receptors Christian Obinger Charlotte Brey Developing rcSso7d-based inhibitory receptors for improving the specificity and safety of NKG2D-CAR-T cells Christian Obinger Benjamin Salzer Structure⁄function relationships in the electron transfer reactions of cellobiose dehydrogenase Dietmar Haltrich Georg Schütz Divergent evolution of the CDE superfamily − analysis of the structural and functional relationship between chlorite dismutases and dye-decolorizing peroxidases Christian Obinger Vera Pfanzagl Recreation of ancestral members of the GMC family of FAD-dependent oxidoreductases Dietmar Haltrich Leander Sützl Development of genetic tools for Selenomonas sputigena to unravel mechanisms of dental plaque colonization Christina Schäffer Susanne Bloch Investigation of cellobiose dehydrogenase and lytic polysaccharide monooxygenase for application in industry Dietmar Haltrich Marita Preims Structural and functional characterisation of a novel bacterial peroxidase with homology to human heme peroxidases Christian Obinger Andrea Nicolussi Structural and functional characterization of Fcab-antigen interaction Christian Obinger Elisabeth Lobner Comparative structural and functional studies on chlorite dismutases and DyP-type peroxidases Christian Obinger Irene Schaffner Human peroxidasins − new multidomain oxidoreductases in the vascular system Christian Obinger Monika Soudi Biocatalysis of chlorite dismutation − functional and structural studies on new peculiar heme enzymes of high practical relevance for bioremediation Christian Obinger Stefan Hofbauer New generations of monoclonal antibodies based on the Fc-fragment of IgG1 − loop engineering and library design of immunoglobulin domains Christian Obinger Christoph Hasenhindl Engineering the interface of Fab fragments Christian Obinger Sylvia Dietrich Functional studies on catalase-peroxidases and design of mechanism-based inhibitors Christian Obinger Bernhard Gasselhuber Directed Evolution of a Beta-Galactosidase into a Transgalactosidase Dietmar Haltrich Barbara Geiger Probing the active site of pyranose dehydrogenase − covalent attachment of the FAD and vicinity of the isoalloxazine Dietmar Haltrich Michael Graf Engineering of a novel fungal esterase by means of directed evolution and site-directed mutagenesis Dietmar Haltrich Daniel Kracher Engineering of pyranose oxidoreductases for modified oxygen reactivity Dietmar Haltrich Iris KrondorferDagmar Brugger Establishing platforms for the production of recalcitrant proteins for functional and structural studies Dietmar Haltrich Georg Mlynek Evolution and engineering of pyranose dehydrogenase for biofuel cell applications Dietmar Haltrich Christoph Gonaus Development of food-grade gene expression vectors in lactic acid bacteria Dietmar Haltrich Minh Nguyen Assembly and interaction mechanisms of S−layer glycoproteins for applications in nanobiotechnology Paul Messner Zoe Megson Carbohydrate−active enzymes in S−layer protein glycosylation Paul Messner Valentin Friedrich Biochemical and Molecular Analysis of the Cell Wall Glyco(prote)ome of Lactobacillus buchneri Paul Messner Eva Smolar Glycoprofiling of oral pathogens – identification of novel glycoproteins and impact of glycosylation on biofilm formation Christina Schäffer Cornelia Rath S−layer mediated display of customized glycans enabled by glycosylation engineering Christina Schäffer Julia Anzengruber Glycoprofiling of the oral pathogen Tannerella forsythia for identifying novel strategies in pathogenicity Christina Schäffer Markus Tomek
