Projects of Area 1 - Structure-function analysis, engineering and design of proteins

List of ongoing PhD research projects:

Novel Lactate-Oxidizing Enzymes for Biosensor Applications   Dietmar Haltrich Lidiia Tsvik
Biochemistry of firmicute coproporphyrin ferrochelatase from Listeria monocytogenes   Christian Obinger Thomas Gabler
Secondary cell wall polymer pyruvylation as an essential ligand modification for SLH-domain recognition   Christina Schäffer Cordula Stefanovic
Engineering a small molecule regulated heterodimerization system for the control of CAR T cells   Christian Obinger Elise Sylvander
Development of a novel binder scaffold   Christian Obinger Magdalena Teufl
The cancer immunotherapy antigen CD19 and its molecular interaction network   Christian Obinger Jacqueline Seigner
Resolving dynamic protein conformations in multidomain enzymes with SAXS   Roland Ludwig Bettina Motycka
Increasing thermostability of pyranose oxidase by enzyme evolution   Dietmar Haltrich Anja Kostelac
Cytochromes as electron transfer mediators for bioelectrodes   Roland Ludwig Lorenz Schwaiger
Allosteric molecular recognition to improve tumor specificity   Christian Obinger Markus Dobersberger
Bispecific antibodies with monovalent antigen engagement in the Fc domain   Florian Rüker Filippo Benedetti
Accessory Activities 3: Novel Flavoprotein Oxidoreductase from bacterial sources   Clemens Peterbauer Virginia Ludovika
Routing the flow of electron from cytochromes to oxidoreductases   Roland Ludwig Franziska Schachinger
Biochemistry of actinobacterial coproheme decarboxylase (ChdC) from Corynebacterium diphteriae   Christian Obinger Hanna Michlits
Structural and mechanistic studies on chlorite dismutase (Cld) from Cyanothece sp. PCC7425   Christian Obinger Daniel Schmidt
Structural characterization of Z-disk complex   Dietmar Haltrich Antonio Sponga
Development of high-throughput-screening of flavin- and heme-dependent oxidoreductases using genetically encoded redox sensors   Clemens Peterbauer Peter Herzog
Extracellular vesicles as tool for drug delivery   Florian Rüker Stefan Vogt

List of completed PhD research projects:
Mechanism and dynamics of extracellular cellulolytic protein interactions   Roland Ludwig Christophe Laurent
Structure−function analysis of a phylum-wide conserved glycosyltransferase central to a novel protein O−glycosylation pathway   Christina Schäffer Matthias Braun
Molecular basis of pyruvylated cell wall polymer – SLH-protein interactions   Christina Schäffer Fiona Hager
Engineering of T-cell receptor for bispecificity   Florian Rüker Flavio Sadio
Biochemistry of invertebrate peroxidasins   Christian Obinger Benjamin Sevcnikar
Developing rcSso7d-based chimeric antigen receptors   Christian Obinger Charlotte Brey
Developing rcSso7d-based inhibitory receptors for improving the specificity and safety of NKG2D-CAR-T cells   Christian Obinger Benjamin Salzer
Structure⁄function relationships in the electron transfer reactions of cellobiose dehydrogenase   Dietmar Haltrich Georg Schütz
Divergent evolution of the CDE superfamily − analysis of the structural and functional relationship between chlorite dismutases and dye-decolorizing peroxidases   Christian Obinger Vera Pfanzagl
Recreation of ancestral members of the GMC family of FAD-dependent oxidoreductases   Dietmar Haltrich Leander Sützl
Development of genetic tools for Selenomonas sputigena to unravel mechanisms of dental plaque colonization   Christina Schäffer Susanne Bloch
Investigation of cellobiose dehydrogenase and lytic polysaccharide monooxygenase for application in industry   Dietmar Haltrich Marita Preims
Structural and functional characterisation of a novel bacterial peroxidase with homology to human heme peroxidases   Christian Obinger Andrea Nicolussi
Structural and functional characterization of Fcab-antigen interaction   Christian Obinger Elisabeth Lobner
Comparative structural and functional studies on chlorite dismutases and DyP-type peroxidases   Christian Obinger Irene Schaffner
Human peroxidasins − new multidomain oxidoreductases in the vascular system   Christian Obinger Monika Soudi
Biocatalysis of chlorite dismutation − functional and structural studies on new peculiar heme enzymes of high practical relevance for bioremediation   Christian Obinger Stefan Hofbauer
New generations of monoclonal antibodies based on the Fc-fragment of IgG1 − loop engineering and library design of immunoglobulin domains   Christian Obinger Christoph Hasenhindl
Engineering the interface of Fab fragments   Christian Obinger Sylvia Dietrich
Functional studies on catalase-peroxidases and design of mechanism-based inhibitors   Christian Obinger Bernhard Gasselhuber
Directed Evolution of a Beta-Galactosidase into a Transgalactosidase   Dietmar Haltrich Barbara Geiger
Probing the active site of pyranose dehydrogenase − covalent attachment of the FAD and vicinity of the isoalloxazine   Dietmar Haltrich Michael Graf
Engineering of a novel fungal esterase by means of directed evolution and site-directed mutagenesis   Dietmar Haltrich Daniel Kracher
Engineering of pyranose oxidoreductases for modified oxygen reactivity   Dietmar Haltrich

Iris Krondorfer
Dagmar Brugger

Establishing platforms for the production of recalcitrant proteins for functional and structural studies   Dietmar Haltrich Georg Mlynek
Evolution and engineering of pyranose dehydrogenase for biofuel cell applications   Dietmar Haltrich Christoph Gonaus
Development of food-grade gene expression vectors in lactic acid bacteria   Dietmar Haltrich Minh Nguyen
Assembly and interaction mechanisms of S−layer glycoproteins for applications in nanobiotechnology   Paul Messner Zoe Megson
Carbohydrate−active enzymes in S−layer protein glycosylation   Paul Messner Valentin Friedrich
Biochemical and Molecular Analysis of the Cell Wall Glyco(prote)ome of Lactobacillus buchneri   Paul Messner Eva Smolar
Glycoprofiling of oral pathogens – identification of novel glycoproteins and impact of glycosylation on biofilm formation   Christina Schäffer Cornelia Rath
S−layer mediated display of customized glycans enabled by glycosylation engineering   Christina Schäffer Julia Anzengruber
Glycoprofiling of the oral pathogen Tannerella forsythia for identifying novel strategies in pathogenicity   Christina Schäffer Markus Tomek